Description
The Human Programmed T cell Death Ligand 1 (Hu PD-L1) ELISA quantitates Hu PD-L1 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant H PD-L1.
Principle of the method
The Human PD-L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen